Gene replacement in gram-negative bacteria: the pMAKSAC vectors.

نویسندگان

  • D Favre
  • J F Viret
چکیده

1.Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580. 2.Ishii, T.M., P. Zerr, X.M. Xia, C.T. Bond, J. Maylie and J.P. Adelman. 1998. Site-directed mutagenesis. Methods Enzymol. 293:53-71. 3.Kirsch, R.D. and E. Joly. 1998. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids Res. 26:1848-1850. 4.Kunkel, T.A. 1985. Rapid isolation and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492. 5.Nelson, M. and M. McClelland. 1992. Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes. Methods Enzymol. 216:279-303. 6.Papworth, C., J.C. Bauer, J. Braman and D.A. Wright. 1996. Site-directed mutagenesis in one day with >80% efficiency. Strategies 9:3-4. 7.Sambrook, J., E. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY. 8.Scott, B., K. Nelson, J. Cline and K. Kretz. 1994. High-fidelity PCR amplification with Pfu DNA polymerase. Strategies 7:62-63. 9.Weiner, M.P., G.L. Costta, W. Schoettlin, J. Cline, E. Mathur and J.C. Bauer. 1994. Sitedirected mutagenesis of double-stranded DNA by the polymerase chain reaction. Gene 151:119-123.

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عنوان ژورنال:
  • BioTechniques

دوره 28 2  شماره 

صفحات  -

تاریخ انتشار 2000